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Test Code 468 Vancomycin A Resistance Gene rPCR

Important Note

Hydrocortisone cream (1% hydrocortisone) and PeptoBbismol (1-5% bismuth subsalicylate) may interfere with testing. Test results may also be affected by concurrent antibiotic therapy, or the number of organisms in the specimen may be below the limit of detection for the test. Mutations or polymorphisms in primer or probe binding regions may affect detection of new or unknown Van A variants resulting in a false negative result. This assay detects Van gene only, not microorganisms; therefore, Van A genes carried by non enterococci, such as Vancomycin-Resistant Staphylococcus aueus strains, may also give a positive result.

Additional Codes

MLAB: VANAPCR

PowerChart: Vancomycin Resistance Rapid PCR

Epic: LAB3440 Vancomycin A Gene RPCR

Test Method

Rapid Polymerase Chain Reaction (rPCR)

Specimen Requirement

Rectal swab collected using the Cepheid sample collection device.

Additional Specimen Collection Information

Collect a rectal swab using the Cepheid sample collection device. The specimen swabs are placed back in the plastic transport tube. Label the tube with patient name and two identifers (patient ID number, DOB, etc.) [include specimen source and date and time of collection]. Transport to the laboratory as soons as possible after collection. Specimens can be kept at room temperature for up to 24 hours. Specimens are stable for 5 days when kept refrigerated.

Performance

Testing is performed Monday-Sunday.

Routine: Typically completed within 4 hours after the specimen arrives at the testing site.

STAT: Typically completed within 2 hours after the specimen arrives at the testing site.

Reference Values

Negative

CPT Code Information

87500 Detection of Infectious Agents by Probe Techniques; Vancomycin resistance, amplified probe technique

Useful For

Detection of vancomycin resistant bacteria from clincal specimen

LOINC Code Information

48814-8 Bacterial Vancomycin Resistance (vanA) Gene [Presence] By Probe And Target Amplification Method