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Test Code HPFH Hemoglobin F, Red Cell Distribution, Blood

Additional Codes

PowerChart: Hemoglobin F

Reporting Name

Hemoglobin F, Red Cell Distrib, B

Useful For

Distinguishing large deletional hereditary persistence of fetal hemoglobin from other conditions with increased percentage of fetal hemoglobin


Determining the distribution of Hb F within red blood cells

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type

Whole Blood EDTA

Advisory Information

This test is a flow cytometry assay that determines the distribution of hemoglobin F (Hb F) within red blood cells. The HGBCE / Hemoglobin Variant, A2 and F Quantitation, Blood test is intended for monitoring purposes, such as the increase in Hb F after therapy, or the levels of hemoglobin variants after transfusion.


This test should not be ordered for fetal-maternal bleed. If fetal maternal bleed assessment is desired, see FMB / Fetomaternal Bleed, Flow Cytometry, Blood.

Necessary Information

1. Indicate the percent of hemoglobin F detected by hemoglobin electrophoresis. If this is not indicated, we will perform HBF / Hemoglobin F, Blood.

2. Indicate the reason for testing.

Specimen Required

Container/Tube: Lavender top (EDTA)
Specimen Volume:
3 mL

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Whole Blood EDTA Refrigerated 7 days

Reference Values

Reported as heterocellular or homocellular

Day(s) and Time(s) Performed

Monday through Saturday; continuously

Test Classification

This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
HPFH Hemoglobin F, Red Cell Distrib, B In Process


Result ID Test Result Name Result LOINC Value
8270 Hemoglobin F, Red Cell Distrib, B 4579-9
2104 Interpretation 59466-3

Reject Due To


Mild reject; Gross reject


Mild OK; Gross OK





Testing Algorithm

The percent of the patient's hemoglobin F is very important to the clinical utility of this test. HBF / Hemoglobin F, Blood will be performed at an additional charge to determine if Hemoglobin F, Red Cell Distribution testing is warranted. If the hemoglobin F value is normal for age or if the value is less than 5%, the red cell distribution test will not be performed. Samples with hemoglobin F (Hb F) levels above 35% will be accepted but are discouraged due to decreased test specificity. For these markedly elevated Hb F levels, a hemoglobin electrophoresis profile with request for beta globin gene molecular testing for elevated Hb F levels is recommended.


For information on thalassemias and appropriate test ordering, see Thalassemia/Hemoglobinopathy Patient Information in Special Instructions.

Method Name

Flow Cytometry

Additional Tests

Test ID Reporting Name Available Separately Always Performed
HBF Hemoglobin F, B No Yes


If not ordering electronically, complete, print, and send a Benign Hematology Test Request Form (T755) with the specimen (