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Test Code V01 Veterinary CBC with Differential

Test Method

Sysmex XN and XS Systems:

  • WBC:  Flow cytometry
  • RBC:  Impedance counting
  • Platelet Count: Impedance counting
  • Platelet F Count: Flow cytometry fluorescence count (performed when appropriate)
  • HGB:  Converted to SLS-hemoglobin and read photometrically.
  • MCV:  The average volume of individual erythrocytes derived from the RBC histogram.
  • RDW:  The size distribution spread of the erythrocytes population derived from the RBC histogram.
  • MPV:  The average volume of individual platelets derived from the PLT histogram.
  • HCT: Measured as a ratio of the total RBC volume to whole blood using cumulative pulse height detection.
  • MCH, MCHC:  Calculated parameters.
    MCH: Weight of HGB  in the average RBC. MCH = HGB ÷ RBC x 10
    MCHC: Average weight of HGB in a measured dilution. MCHC = HGB ÷ HCT x 100
  • WBC Differential: The instrument makes 3 measurements (volume, conductivity and scatter) as each cell passes through the flow cell. The low frequency impedance measurement defines cell volume. The high frequency conductivity measurement indicates the internal conductivity. The light scatter measurement indicates the structure and shape. An algorithm is applied to determine different cell populations.
  • Abs Neutrophils, Abs Lymphocytes, Abs Monocytes, Abs Eosinophils, Abs Basophils:
    Calculated Parameters [Total WBC x (%Diff Analyte Result/100)] = Absolute Diff Analyte Count

Specimen Requirement

EDTA whole blood is required.

Additional Specimen Collection Information

Whole blood collected in EDTA purple-top tube. The tube must be at least one quarter full to be acceptable for testing.

Capillary specimen collected in EDTA microtube. The  tube must contain at least 375 mcL (bottom line) to be acceptable for testing.

Performance

Testing is performed Monday through Sunday.

Routine orders: Typically completed within 4 hours after the specimen arrives at the testing site.

Expedite orders: Typically completed within 1 hour after the specimen arrives at the testing site.

STAT orders: Typically completed within 30 minutes after the specimen arrives at the testing site.

Reference Values

Canine:*

  • RBC: 5.5-8.5 x 106/mcL
  • Hemoglobin: 12-18 g/dL (8-12 g/dL dogs 5-6 wks old; gradual increase to adult levels by 5 mo.)
  • Hematocrit: 37-55%  (25-37% dogs 5-6 wks old; gradual increase to adult levels by 5 mo.)
  • MCV: 60-77 fL
  • MCH: 19.5-30 pg
  • MCHC: 32-36 g/dL
  • Platelets: 200-500 x 103/mcL
  • WBC: 6.0-17.0 x 103/mcL
  • Neutrophils: 3.0-11.4
  • Lymphocytes: 1.0-4.8
  • Monocytes: 0.15-1.35
  • Eosinophils: 0.1-0.75
  • Basophils: 0.0-0.1

Feline:*

  • RBC: 5.0-10.0 x 106/mcL
  • Hemoglobin: 10-15 g/dL (8-11 g/dL cats 5-6 wks old; gradual increase to adult levels by 5 mo.)
  • Hematocrit: 30-45%  (24-34% cats 5-6 wks old; gradual increase to adult levels by 5 mo.)
  • MCV: 39-55 fL
  • MCH: 12.5-17.5 pg
  • MCHC: 30-36 g/dL
  • Platelets: 300-800 x 103/mcL
  • WBC: 5.5-19.5 x 103/mcL
  • Neutrophils: 2.5-12.5
  • Lymphocytes: 1.5-7.0
  • Monocytes: 0.0-0.85
  • Eosinophils: 0.0-0.75
  • Basophils: 0.0-0.1

Other: Reference values are not established for non-human species.

 

*Iowa State University College of Veterinary Medicine. Reference Intervals. 6/17/2017

CPT Code Information

Not applicable

Useful For

Screening tool to confirm a hematologic disorder.

Establish or rule out a diagnosis.

Detect an unsuspected hematologic disorder.

These counts are used as clinical guides in the diagnosis and/or monitoring of many diseases.

LOINC Code Information

Not applicable