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Test Code 042 Fetal Hemoglobin Stain (Kleihauer-Betke Test)

Additional Codes


PowerChart: Fetal HGB Kleihauer Betke

Epic: LAB762 Kleihauer Betke Stain

MA Cerner: Fetal Hemoglobin (K/B)

Test Method

Kleihauer Bedtke Staining Technique, Fetal Hemoglobin Kit; Simmler

Specimen Requirement

1 mL maternal whole blood specimen is required.

Additional Specimen Collection Information

Collect maternal blood in EDTA purple-top. Collect as soon as possible after delivery or prior to birth if a fetal bleed is suspected. The minimum specimen required is 1 mL. Refrigerate the specimen until analysis; analyze promptly.


Testing is performed Monday through Sunday.

Typically completed within 24 hours after the specimen arrives at the testing site.


This test is performed by a pathologist.

Reference Values

Interpretation by a pathologist.

CPT Code Information

85460 Coagulopathy Testing: Hemoglobin or RBCs, fetal, for fetomaternal hemorrhage; differential lysis (Kleihauer-Betke)

Useful For

Determining the volume of fetal-to-maternal hemorrhage for the purposes of recommending an increased dose of the Rh immune globulin

LOINC Code Information

14276-0 Hemoglobin F Distribution [Interpretation] In Blood By Kleihauer-Betke Method